REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal (SAP7F407) anti-bassoon	Novus Biologicals	Cat. # NB120-13249; RRID: AB_788125
Rabbit polyclonal anti-Homer1	Synaptic Systems	Cat. #1 60003; RRID: AB_887730
Guinea pig polyclonal anti-GLT-1	Merck	Cat. # AB1783; RRID: AB_90949
Donkey anti-mouse IgG	Biotium	Cat. # 20105; RRID: AB_10557030
Goat anti-rabbit IgG	Rockland	Cat. #6 11-152-122S; RRID: AB_10893832
Donkey anti-guinea pig IgG	Jackson ImmunoResearch Labs	Cat. # 706-606-148; RRID: AB_2340477
Bacterial and Virus Strains
AAV9.hSynap.iGluSnFr.WPRE.SV40	Penn Vector Core; Marvin et al., 2013	Addgene Cat. # 98929-AAV9
AAV5.GfaABC1D.Pi.lck-GFP.SV40	Penn Vector Core	Addgene Cat. #105598-AAV5
AAV5.GfaABC1D.Pi.lck-GCaMP6f.SV40	Penn Vector Core	Addgene Cat. # 52924-AAV5 Batch: CS0846L
AAV GFAP-iGluSnFR	Penn Vector Core	Addgene Cat. # 44332-AAV5
AAV5.GfaABC1D.cyto-tdTomato.SV40	Penn Vector Core; Shigetomi et al., 2013	Cat:AV-5-PV3106; Lot: V5606L; RRID: Addgene_44332
AAV9.Syn.GCaMP6f.WPRE.SV40	Penn Vector Core	Cat: AV-9-PV2822; Lot: CS0932; RRID: Addgene_100837
Chemicals, Peptides, and Recombinant Proteins
DNI-GLU-TFA	Femtonics	DNI-GLU-TFA
Picrotoxin	Tocris	Cat. # 1128
CGP 52432	Tocris	Cat. # 1246/10
Bumetanide	Tocris	Cat. # 3108
Biocytin	Sigma-Aldrich	Cat. # B4261
NMDA	Tocris	Cat. # 0114/50
Biotin-tag, Synthetized as double stranded DNA	Epoch Life Science	PinPoint™ Xa-1 (Promega #V2031)
pRSET FLIPE-600n	Wolf B. Frommer	Addgene #13537
pRSET bFLIPE600n	This paper	N/A
Sulfo-NHS EZ Link Biotin	ThermoFisher	Cat. #21217
Streptavidin, unconjugated	ThermoFisher	Cat. #SNN1001
(+)-MK-801 hydrogen maleate	Sigma Aldrich	Cat. #M107
D-APV	Abcam	Cat. #ab120003
NBQX disodium salt	Abcam	Cat. #ab120046
Ro 25-6981 maleate salt	Abcam	Cat. #ab120290
S3 Fragment, ADF/cofilin	Anaspec	Cat. #AS-62637
Oregon Green 488 BAPTA-1	ThermoFisher	Cat. #O6806
Picrotoxin	Tocris	Cat. #1128
CGP52432	Tocris	Cat. #1246
MNI-caged-L-glutamate	Bio-Techne Ltd	Cat-#1490
DL-AP5	Bio-Techne Ltd	Cat. # 0105
CGP 52432	Bio-Techne Ltd	Cat. # 1246
NBQX disodium salt	Bio-Techne Ltd	Cat. # 1044
TFB-TBOA	Bio-Techne Ltd	Cat. # 2532
Tetrodotoxin citrate	Bio-Techne Ltd	Cat. # 1069
WIN 55,212-2 mesylate	Bio-Techne Ltd	Cat. # 1038
DHPG	Bio-Techne Ltd	Cat. # 0805
Muscimol	Bio-Techne Ltd	Cat. # 0289
MK 801	Bio-Techne Ltd	Cat. # 0924
Chonditinase ABC	Sigma-Aldrich	Cat-# C3667
nEphrin-A4/FC Chimera	Sigma-Aldrich	Cat. # E0403
Fc Fragment	Sigma-Aldrich	Cat. # AG714
TGN-020	Sigma-Aldrich	Cat. # SML0136
Bumetanide	Sigma-Aldrich	Cat. # B3023
Alexa Fluor-594 Hydrazide	ThermoFisher Scientific	Cat.# A10438
Alexa Fluor-488 Hydrazide	ThermoFisher Scientific	Cat.#A10436
Fluo-4, Pentapotassium Salt	ThermoFisher Scientific	Cat.# F14200
Oregon Green 488 BAPTA-1, Hexapotassium Salt	ThermoFisher Scientific	Cat.# O6806
NPE-Caged Ins 1,4,5-P3	ThermoFisher Scientific	Cat. # I23580
Antisedan	Vetoquinol	Cat. # 459180
Carprofen Rimadyl	Zoetis	N/A
Forskolin	Sigma-Aldrich	Cat. # F6886/3917
VECTASTAIN® ABC-HRP Kit	Vector labs, USA	Cat. #PK-4000
Picrotoxin	Sigma-Aldrich	Cat. #P1675; CAS: 124-87-8
Forskolin	Cayman Chemical	Cat. #11018; CAS: 66575-29-9
Rolipram	Cayman Chemical	Cat. #31111; CAS: 85416-75-7
NaBH4	Sigma-Aldrich	Cat. #71320; CAS: 16940-66-2
PBS	Sigma-Aldrich	Cat. #4417
CuSO4	Sigma-Aldrich	Cat. #C8027; CAS: 7758-99-8
NH4Cl	Sigma-Aldrich	Cat. #254134; CAS: 12125-02-9
Saponin	Bio Basic	Cat. #SB4521; CAS: 8047-15-2
BSA	Sigma-Aldrich	Cat. #A7906 CAS: 9048-46-8
Urea	Sigma-Aldrich	Cat. #U6504; CAS: 57-13-6
Glycerol	Fisher Scientific	Cat. #BP229-1 CAS: 56-81-5
Triton X-100	Sigma-Aldrich	Cat. #T9284; CAS: 9002-93-1
Agarose	Sigma-Aldrich	Cat. #9539; CAS: 9012-36-6
Catalase	Sigma-Aldrich	Cat. #C40; CAS: 9001-05-2
glucose oxidase	Sigma-Aldrich	Cat. #G2133; CAS: 9001-37-0
glucose	Sigma-Aldrich	Cat. #G8270; CAS: 50-99-7
KCl	Sigma-Aldrich	Cat. #P9333; CAS: 7447-40-7
MEA-HCl	Sigma-Aldrich	Cat. #M6500; CAS: 56-57-0
PFA	Sigma-Aldrich	Cat. #P6148; CAS: 30525-89-4
TCEP	Sigma-Aldrich	Cat. #C4706; CAS: 51805-45-9
Tris base	Sigma-Aldrich	Cat. #33742; CAS: 77-86-1
DAB	Sigma-Aldrich	Cat. #D5905-50TAB
Experimental Models: Organisms/Strains
Mouse: C57BL/6J	Charles River Laboratories	C57BL/6NCrl Strain: 0159
Mouse: C57BL/6J	Charles River UK	RRID: IMSR_JAX:000664
Rat: Sprague-Dawley	Charles River Laboratories	Crl:CD (SD) Strain: 0204
Transgenic mouse line hGFAP-EGFP	Frank Kirchoff; Nolte et al., 2001	MGI ID:6188855
Wistar rats	Charles River Laboratories	Strain Code: 003
Mouse: Aqp4flox/flox. Strain background: C57BL/6J	Ole Petter Ottersen; Haj-Yasein et al., 2011	N/A
Thy1-YFP-H: B6.Cg-Tg(Thy1-YFPH)2Jrs/J	The Jackson Laboratory	Stock No: 003782
Software and Algorithms
ImageJ	NIH	RRID:SCR_003070; https://imagej.nih.gov/ij/
pClamp10	Molecular Devices	RRID: SCR_011323; https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite
OriginPro	OriginLab Inc	RRID: SCR_014212; https://www.originlab.com/origin
MES 4.x-5.x	Femtonics Ltd.	RRID:SCR_018309; https://uk.mathworks.com/products/connections/product_detail/femtonics-mes.html
Imspector Image Acquisition & Analysis Software v0.1	Abberior Instruments Development Team	RRID:SCR_015249; https://imspectordocs.readthedocs.io/en/latest/intro.html
Huygens STED Deconvolution Software	Huygens Professional (SVI)	RRID:SCR_014237; https://svi.nl/Huygens-Deconvolution
WinWCP Versions 4.x-5.x	Strathclyde Electrophysiology Software	RRID:SCR_014270; http://spider.science.strath.ac.uk/sipbs/software_ses.htm
SymPhoTime 64	PicoQuant	RRID:SCR_016263; https://www.picoquant.com/products/category/software/symphotime-64-fluorescence-lifetime-imaging-and-correlation-software
SEM. Align 1.26b	John Fiala, Boston University	https://synapseweb.clm.utexas.edu
Trace 1.26b	John Fiala, Boston University	https://synapseweb.clm.utexas.edu
Autodesk 3D studio Max 8	Autodesk	https://www.autodesk.com
MATLAB	Mathworks	RRID:SCR_001622; https://uk.mathworks.com/products/matlab.html
Other
Multiclamp 700B	Molecular Devices	RRID:SCR_018455
Olympus FluoView1000	Olympus	RRID:SCR_014215
Femto2D	Femtonics	Femto2D
Femto3D RC	Femtonics	Femto3D RC
BioRad Radiance 2100	BioRad	Radiance 2100