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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: J Mol Cell Cardiol. 2020 Sep 18;149:27–40. doi: 10.1016/j.yjmcc.2020.09.004

Figure 3: Overexpression of Pyk2 increases Cx43 phosphorylation at Y247, Y265, and Y313.

Figure 3:

(A) HEK 293T cells were co-transfected with c-SrcY530F (active c-Src; 24 hr) and Pyk2 wild type (WT) or a Pyk2 kinase dead (KD) mutant (K457A) and lysate was Western blotted. Antibodies used are labeled on the left of each panel. (B) Protein levels were quantified by analyzing scanned blots using ImageJ software, with normalization of protein expression to the control lane (value set arbitrarily as 1). Phosphorylated protein levels were normalized to total protein. Data are representative of three independent experiments (one-way ANOVA, *P<0.05, **P<0.01).