TABLE 2.
List of Primary Antibodies used for Immunolabeling Human Tissue
| Antigen (protein abbreviation) | Recognized Cells | Manufacturer (catalog #) | Host | Dilution |
|---|---|---|---|---|
| α-smooth muscle actin (ASMA) | smooth muscle cells | Sigma-Aldrich, Vienna, Austria | mouse monoclonal | 1:400 |
| Cluster of differentiation 31 (CD31) | endothelial cells | Invitrogen/Thermo, Fisher Vienna, Austria | mouse monoclonal | 1:50 |
| Cluster of differentiation 34 (CD34) | endothelial cells | Reliatech, WolfenbÜttel, Germay | mouse monoclonal | 1:200 |
| Cluster of differentiation 68 (CD68) | macrophages | Dako, Vienna, Austria | mouse monoclonal | 1:50 |
| Cluster of differentiation 146 (CD146) | endothelial cells | Invitrogen/Thermo Fisher, Vienna, Austria | mouse monoclonal | 1:500 |
| Desmin (DES) | pericytes | Santa Cruz, Heidelberg, Germany | goat polyclonal | 1:200 |
| Ionized calcium-binding adapter molecule 1 (IBA1) | microglia | Abcam, Cambridge, UK | goat polyclonal | 1:500 |
| Lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) | lymphatic endothelial cells | Reliatech, WolfenbÜttel, Germay | mouse monoclonal | 1:800 |
| Protein-gene product 9.5 (PGP9.5) | pan neurons | Sigma-Aldrich, Vienna, Austria | guinea pig polyclonal | 1:400 |
| Retinaldehyde dehydrogenase 2 (RALDH2) | different cell types | Sigma-Aldrich, Vienna, Austria | rabbit polyclonal | 1:750 |
| Vimentin (VIM) | mesenchymal cells | Dianova, Hamburg, Germany | mouse monoclonal | 1:500 |