Activation of Wnt/β-catenin signaling induces a transcriptional program that promotes zG differentiation, which includes several genes that support the production of aldosterone (e.x. Agtr1, Cyp11b2, Nr4a1, and Nr4a2). Simultaneously, Wnt/β-catenin activation represses zF identity through mechanisms that include direct up regulation of Pde2a. Conversely, cAMP/PKA signaling promotes zF differentiation by increasing expression of several genes that support the production of glucocorticoids (e.x. Mc2r, Cyp11b1, Star, Cyp11a1, and Akr1b7). PKA signaling additionally represses Wnt/β-catenin activation through mechanisms that include inhibition of Wnt4. The balance between Wnt and PKA signaling in part determines adrenocortical cell fate. Schematic created with BioRender.com.