Table 3.

Evaluation of phagocytosis, respiratory burst, and metabolic activity of RAW 264.7 cells following exposure to cell mitogens.

Formulae	Cell bound and internalized SPA (%)	Internalized SPA (%)	Membrane attached SPA (%)	Respiratory burst (%)	Metabolic activity (%)
24 h exposition
ConA	68.88 ± 0.14***	49.45 ± 0.06***	19.43 ± 0.16**	53.63 ± 0.02***	24.84 ± 0.06**
PWM	62.20 ± 0.16***	43.51 ± 0.13***	18.69 ± 0.24	57.62 ± 0.03***	26.44 ± 0.06***
PHA	48.08 ± 0.05*	40.51 ± 0.20***	7.56 ± 0.16***	67.46 ± 0.04***	22.80 ± 0.02***
LPS	54.88 ± 0.26***	43.17 ± 0.22***	11.71 ± 0.26***	70.37 ± 0.22***	30.14 ± 0.13***
48 h exposition
ConA	52.37 ± 0.24*	38.23 ± 0.11***	14.14 ± 0.13***	41.39 ± 0.12	22.35 ± 0.16***
PWM	52.38 ± 0.24*	32.25 ± 0.20**	20.13 ± 0.19**	46.35 ± 0.15***	21.33 ± 0.12***
PHA	35.16 ± 0.25***	30.90 ± 0.15***	4.26 ± 0.17***	47.22 ± 0.21***	30.20 ± 0.12**
LPS	44.23 ± 0.19***	35.88 ± 0.11***	8.35 ± 0.27***	63.23 ± 0.13***	38.79 ± 0.26***

Phagocytic activity, oxidative burst, and metabolic activity were expressed as percentage of cells actually undergoing these processes in a population of 10,000 cells. All data are presented as mean ± SD. Tests were carried out in triplicate. Statistical significance of differences between untreated and stimulated cells using one-way ANOVA and post-hoc Bonferroni tests is expressed as:

^***P < 0.001,

^**0.001 < P < 0.01,

^*0.01 < P < 0.05.