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. Author manuscript; available in PMC: 2020 Dec 15.
Published in final edited form as: J Bone Miner Res. 2020 Sep 19;35(12):2432–2443. doi: 10.1002/jbmr.4142

Figure 1. Effect of various anti-glycolytic agents on osteoprogenitors.

Figure 1.

(A) Anti-glycolytic agents including 2-deoxyglucose (2DG), dichloroacetate (DCA), 3-bromopyruvate (3-BP), and sodium oxamate (OXA) were used to target various steps of the glycolytic pathway. (B) Undifferentiated C3H10T1/2 cells were treated with PBS as a vehicle control or various indicated doses of 2DG, DCA, 3-BP, or OXA for 48 h. Cells were then stained with Calcein AM at 1 μM for 30 min to assess viability. Only OXA did not cause any toxicity at the studied concentrations. (C) C3H10T1/2 cells were osteoinduced for 14 days in medium containing ascorbic acid and ß-glycerol phosphate and then treated with OXA for 48 h. Calcein AM staining revealed no significant negative effect of OXA on the viability of osteoinduced C3H10T1/2 cells. Data are presented as boxplots with actual data points, median, and range. P value was determined by ANOVA shown in data sets with p < 0.05.