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VLP surface immobilization and imaging. (A) Schematic of VLP-surface attachment strategy (bottom to top): glass surfaces were functionalized with Biotin-PEG-Silane, then neutravidin, then biotinylated anti-S antibody which then allowed for the capture of purified VLPs. VLP imaging showed initially symmetric particles. (B) which developed prominent deformations likely indicative of bursts upon repeated AFM imaging with 1 nN peak force (C).
