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. 2020 Dec 14;10:21881. doi: 10.1038/s41598-020-78930-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparative analysis of the metabolites oriented by area. After visualization of the metabolites by MS imaging, we compared their relative intensities in three separate areas: the ischemic area (average of five dots), the peri-infarct area, and the non-infarct area. (A) A schematic illustration representing the region of interest (ROI) that we used. As a control, we used the contralateral sides of brains from MCAO-vehicle mice. (B) The bar graph represents the average intensity of each ROI. Data are shown as the mean ± the S.E.M. of five mice for each group. * and ** indicate p < 0.05 and p < 0.01 versus the control group, respectively, and # and ## indicate p < 0.05 and p < 0.01 versus the MCAO-vehicle group, respectively.