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. 2020 Dec 14;10:21873. doi: 10.1038/s41598-020-78788-z

Figure 2.

Figure 2

Effects of dual PI3Kα and mTOR inhibition in GBM spheroids. (A) U87 GBM cells were grown as spheres in CSC medium for at least 5 days. Spheres were dissociated and seeded at 500 cells/well into round-bottom 96-well plates in the presence of increasing concentrations of alpelisib as indicated. After 7 days, spheres were stained with acridine orange and imaged to determine cross-sectional area. Data represent means ± SEM of 5 independent experiments, each done in triplicate. Representative images are shown in the top panels. (B, C) Same experiment as in (A) using 5 µM alpelisib and 1 µM OSI-027 (B) or 10 µM alpelisib and 2 µM OSI-027 (C). Data represent means ± SEM of 6 independent experiments, each done in triplicate. Representative images are shown in the top panels. Unpaired one-way ANOVA, *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.