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. 2020 Dec 14;10:217. doi: 10.1186/s13568-020-01164-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Boxplot of the wzi-3 and wzi-4 average melting temperatures. The distributions of wzi-3 and wzi-4 average melting temperatures (aTm) obtained by each instrument (“CFX96” for Biorad CFX96; “Eco_RT” for Illumina Eco Real-Time; “QS_6Flex” for Applied Biosystem QuantStudio 6 Flex) and operator (AP and MP; colored in blue and yellow, respectively) are shown. Boxes range between the 25th and the 75th quartiles, and bold horizontal lines represent the median values