Figure 3.

Functional Characterization of CA3 Pyramidal Cell Responses in Dock10-Cre;Ai32 Slices

(A) Experimental setup for recording postsynaptic CA3 pyramidal cell (PC) responses (B–L).

(B–D) Excitatory postsynaptic currents (EPSCs) in response to 100 × 5 ms light pulses (LPs; 20 Hz, ~5.16 mW/mm²) recorded at three different external calcium concentrations, [Ca²⁺]₀ = 1 mM (B); [Ca²⁺]₀ = 2 mM (C); and [Ca²⁺]₀ = 4 mM (D). Traces in (B) and (C) were recorded from the same cell. The first (bottom left) and last (bottom right) five responses are shown with an expanded timescale.

(E) EPSC responses to the initial (top) and final (bottom) five stimuli in a 100 LP train (20 Hz, ~5.16 mW/mm²) at 2 mM (gray) and 4 mM (black) [Ca²⁺]₀ shown superimposed.

(F) Paired-pulse ratios at different [Ca²⁺]₀ for two LPs (5.16–9.08 mW/mm²) and inter-stimulus intervals (ISI).

(G and H) Normalized EPSC amplitudes for 100 LPs (5.16–9.08 mW/mm²) at 10 Hz (G) and 20 Hz (H) at 1 mM (white, n = 7 each) 2 mM (gray, n = 10 each), and 4 mM (black, n = 11 each) [Ca²⁺]₀.

(I) EPSCs for a single LP (5.16 mW/mm²; [Ca²⁺]₀ = 2 mM) before (top trace) and after (bottom trace) the application of 1 μM TTX.

(J and K) EPSCs in response to 100 LPs (20 Hz; 5.45 mW/mm²; [Ca²⁺]₀ = 2 mM) before (J) and after (K) application of 1 μM TTX. Initial (bottom left) and final (bottom right) responses are shown at expanded timescales.

(L) EPSCs in response to five LPs (20 Hz; 5.45 mW/mm²; [Ca²⁺]₀ = 2 mM) before (solid line) and after (dashed line) application of 2 µM DCGIV.

Error bars indicate mean ± SEM.