Table 1. List of sequences of forward (F) and reverse (R) primers used in PCR.
Genes | Primer sequences |
---|---|
OCT4 | F 5′-TGAGTAGTCCCTTCGCAAGC-3′ |
R 5′-TTAGCCAGGTCCGAGGATCA-3′ | |
NANOG | F 5′-ACCAGAACTGTGTTCTCTTCCACC-3′ |
R 5′-CCATTGCTATTCTTCGGCCAGTTG-3′ | |
RUNX2 | F 5′-GACCAGTCTTACCCCTCCTACC-3′ |
R 5′-CTGCCTGGCTCTTCTTACTGAG-3′ | |
OCN | F 5′-GTGCAGAGTCCAGCAAAGGT-3′ |
R 5′-TCAGCCAACTCGTCACAGTC-3′ | |
C/EBPB | F 5′-TTTGTCCAAACCAACCGCAC-3′ |
R 5′-GCATCAACTTCGAAACCGGC-3′ | |
C/EBPA | F 5′-GCAAACTCACCGCTCCAATG-3′ |
R 5′-CTTCTCTCATGGGGGTCTGC-3′ | |
ACTB | F 5′- GTCATTCCAAATATGAGATGCGT-3′ |
R 5′- GCTATCACCTCCCCTGTGTG-3′ |