Figure 4. Role of ubiquitin proteasome pathway in LNT-mediated degradation of HIF-1α.

(A) Expression of HIF-1α, Nur77, mTOR, VEGF, PARP and PCNA in MCF-7 cells treated with cobalt chloride (0 and 100 μM) in the control group and Nur77-knockdown group. (B) Protein levels of HIF-1α, Nur77, mTOR and VEGF as analyzed by Western blotting in MCF-7 cells treated with MG132 and LNT under normoxic and hypoxic conditions. Abbreviation: LNT, Lentinus edodesr. Data are shown as the means ± SD, n≥3.