Experimental groups, timeline and MGS mRNA expression levels after tamoxifen administration. A. Control and malinKO mice were injected with tamoxifen at either 4 or 6 months of age. Samples were collected at the different time points indicated. B. Quadriceps muscle and brain tissue obtained from 11-month-old wt, malinKO and 4mMGS-KO+malinKO mice-treated with tamoxifen at 4 months of age. Graphics show the MGS mRNA relative expression level (2−ΔΔCt). 18S rRNA was used as housekeeping gene for internal control. Each dot represents one mouse, n: 5–8. Data shown as mean±SD. Statistics: Student’s t-test: *p≤0.05, ** p≤0.005, *** p≤0.001.