Figure 5.

LB quantification in the cortical prefrontal region. A. Cortical LB visualization by immunofluorescence. Representative images of the prefrontal cortex region (Cx) stained with anti-MGS (green, upper panels) and anti-laforin (green, lower panels) antibodies in combination with DAPI (blue) from each group. Scale bar: 100μm. B. Total number of particles detected in Cx from each brain by anti-MGS and anti-laforin antibodies stainings. Mean values between experiments are represented, a total of 3–4 non-consecutive sections were measured per brain for MGS and 4–6 non-consecutive sections for laforin. Each dot represents one mouse. Data are expressed as number of particles per mm². C. Quantification of CAL and nLBs in the prefrontal cortex by MGS staining. Mean values between experiments are represented 3–4 sections were analyzed in each brain n=2–10 mice per group as indicated. Data are expressed as number of particles per mm², shown as mean±SD. In all the graphics, each dot represents one mouse. Statistics: Student’s t-test test validated by by a linear mixed effect model analysis: *p≤0.05, ** p≤0.005, *** p≤0.001.