Figure 6.

Astrogliosis in hippocampus by GFAP immunofluorescence. A. Representative images of the Hp regions of each experimental group stained with anti-GFAP antibody (red) and DAPI (blue). Scale bar: 500μm. B. Quantification of GFAP inmunostaining. The percentage of positive pixel for GFAP staining was quantified and represented as % positive pixel/mm². Mean values between experiments are shown from 3 non-consecutive sections for each sample. Each dot represents one animal, n= 5–10. Data shown as mean±SD. Statistics: Student’s t-test validated by a linear mixed effect model analysis: *p≤0.05, ** p≤0.005, *** p≤0.001.