Table 1.
Oligonucleotides
| Name | Sequence (5′ - 3′) |
|---|---|
| TAQNde1-F | AAGGAGATATACATATGCGCGGCATGCTGCCACT |
| TAQXho1R | GGTGGTGGTGCTCGAGTCATTCCTTGGCACTCAGCCAATCTTC |
| pET-ATG-R | CATATGTATATCTCCTTCTTAAAGTTAAAC |
| Stoff-F | AGCCCAAAAGCGCTG |
| HhH-Stoff-F | AAGGAGATATACATATGAAGTCGGGCCGTCAGGAG |
| HhH-GGG-Stoff-R | GCTTTTGGGCTCATACCTCCGCCTACGTCGTAGGCACCGCGAAGCTTACGTCTGATG |
| StoffAPWP-F | GCCCCGTGGCCACCTC |
| KALEtoLPETGGG-R | TTCGCCTCCACCTGTTTCCGGCAGTGGGCTTTCCAAGAGCCCGAAC |
| SS07DINF-F | GGAGATATACATATGGCAACCGTTAAATTCAAGTATAAGG |
| SSO7DINF-R | ACCTGTTTCCGGCAGACTACCTTTTTTTTGCTTTTCCAGCA |
| EXOLPETV2-F | CTGCCGGAAACAGGTGGAG |
| Nde1-Stoff-F | AAGGAGATATACATATGGGAGGCGAAGCCCCG |
| Xho1-Stoff-R | TTTCTTTACCAGACTCGAGTCATTCCTTGGCAC |
| SSO7D-BAM-pDUET-F2 | ACCACAGCCAGGATCCaGCAACCGTTAAATTCAAGTATAAGG |
| SSO7D-Sort-SalI-pDUET-R | CCGCAAGCTTGTCGACTTATCCTCCAGTCTCAGGCAGGCTCCCACCACCACTACCTTTTTTTTGCTTTTCCAGCATTTGCAG |
| DUETHHH-F | ATAATGCTTAAGTCGAACAGAAAGTAATCGTATTG |
| DUETHHH-R | TCCTCCAGTCTCAGGCAGTACGT |
| SPYCDUET-F2 | CCTGAGACTGGAGGATCTGTCGAC GATTCAGCCACCCACATTAAATTTAGTAAACGCGA |
| SPYCHHH-R2 | CGACTTAAGCATTATGAATTCTTAGCCGTTAACTGTGACCTGTCCCTGTTCATT |
| SPYTINF-TOP | AAGGAGATATACATATGGGAGCTCACATCGTGATGGTGGACGCATATAAGCCGACTAAGGGATCTACTAGTTCTATGGGAGGCGAAGC |
| SPYTINF-B | GCTTCGCCTCCCATAGAACTAGTAGATCCCTTAGTCGGCTTATATGCGTCCACCATCACGATGTGAGCTCCCATATGTATATCTCCTT |
| NanoBigDUET-F | CCTGAGACTGGAGGATCTGTCGACATGGTCTTCACACTCGAAGATTTCGTTGG |
| NanoBigHHH-R | CGACTTAAGCATTATGAATTCTTAACTGTTGATGGTTACTCGGAACAGCATG |
| NanoSmallV2-TOP | AAGGAGATATACATATGGTAACCGGTTACCGTTTGTTCGAAGAGATTTTGGGATCTACTAGTTCTATGGGAGGCGAAGC |
| NanoSmallV2-BTM | GCTTCGCCTCCCATAGAACTAGTAGATCCCAAAATCTCTTCGAACAAACGGTAACCGGTTACCATATGTATATCTCCTT |
| NanoStrongV2-TOP | AAGGAGATATACATATGGTATCAGGTTGGCGTTTGTTCAAGAAGATTAGCGGATCTACTAGTTCTATGGGAGGCGAAGC |
| NanoStrongV2-BTM | GCTTCGCCTCCCATAGAACTAGTAGATCCGCTAATCTTCTTGAACAAACGCCAACCTGATACCATATGTATATCTCCTT |
| NanoStrongAV2-TOP | AAGGAGATATACATATGGTAACCGGTTACCGTTTGTTCGAAAAGATTAGCGGATCTACTAGTTCTATGGGAGGCGAAGC |
| NanoStrongAV2-BTM | GCTTCGCCTCCCATAGAACTAGTAGATCCGCTAATCTTTTCGAACAAACGGTAACCGGTTACCATATGTATATCTCCTT |
| NanoStrongBV2-TOP | AAGGAGATATACATATGAACGTAACCGGTTACCGTTTGTTCAAGAAGATTAGCAACGGATCTACTAGTTCTATGGGAGGCGAAGC |
| NanoStrongBV2-BTM | GCTTCGCCTCCCATAGAACTAGTAGATCCGTTGCTAATCTTCTTGAACAAACGGTAACCGGTTACGTTCATATGTATATCTCCTT |
| NanoStrongCV2-TOP | AAGGAGATATACATATGAACGTATCAGGTTGGCGTTTGTTCAAGAAGATTAGCAACGGATCTACTAGTTCTATGGGAGGCGAAGC |
| NanoStrongCV2-BTM | GCTTCGCCTCCCATAGAACTAGTAGATCCGTTGCTAATCTTCTTGAACAAACGCCAACCTGATACGTTCATATGTATATCTCCTT |
| NanoStrongDV2-TOP | AAGGAGATATACATATGGGTGTAACCGGTTGGCGTTTGTGCGAACGTATTTTGGCCGGATCTACTAGTTCTATGGGAGGCGAAGC |
| NanoStrongDV2-BTM | GCTTCGCCTCCCATAGAACTAGTAGATCCGGCCAAAATACGTTCGCACAAACGCCAACCGGTTACACCCATATGTATATCTCCTT |
| MStoffV2-TOP | AAGGAGATATACATATGGGAGGATCTACTAGTTCTATGGGAGGCGAAGC |
| MStoffV2-BTM | GCTTCGCCTCCCATAGAACTAGTAGATCCTCCCATATGTATATCTCCTT |
| LPETGG-Sal1-F | TGCCTGAGACTGGAGGATCTGTCGAC |
| SPYTR6.2 | CATAGAACTAGTAGATCCCTTAGTCGGCTTATATGCGTCMNNMNNMNNMNNGTGAGCTCCCATATGTATATCTCCTTCTTATACTTAACTAATATAC |
| SPYTR5.2 | CATAGAACTAGTAGATCCCTTAGTCGGMNNMNNMNNGTCCACCATCACGATMNNMNNMNNCATATGTATATCTCCTTCTTATACTTAACTAATATAC |
| SpyC-NNK1-R | CAGTAGCCACTTCATACCCGTCCGGTGCGGCGGTTTCCACMNNGGTGTATTTACCTGGGTACAGGT |
| SpyC-NNK2-F | GACGGGTATGAAGTGGCTACTGCAATTACTNNKACCGTAAATGAACAGGGACAGGTCACAG |
| SpyT-NNK-R | CATAGAACTAGTAGATCCCTTAGTCGGCTTATATGCGTCCACCATCACMNNGTGAGCTCCCATATGTATATCTCCTTCTTATACTTAACTAATATAC |
| INF-Pet22-JW-SSO-SPYC-F | AAGGAGATATACATATGGGCAGCAGCCATCACCATCATCACC |
| INF-Pet22-JW-SSO-SPYC-R | GACGGAGCTCGAATTCTTAGCCGTTAACTGTGACCTGTCCCTGTTCATTTAC |
| INF-Pet22-JW-SPYT-F | AAGGAGATATACATATGGGAGCTCACATCGTGATGGTGGACGC |
| INF-Pet22-JW-SPYT-R | GGTGGTGGTGCTCGAGTTCCTTGGCACTCAGCCAATCTTCGCC |
| BIOOLS79-duetMCS2-F | BIOTIN-GTAAGCTGGAAGTTGTTGCTGCGTGAGCGGATAACAATTCCCCATCTTAG |
| SpyTag-SpeI-R2 | TTCGCCTCCCATAGAACTAGTAGATCC |
| BIO-OLS79-LPETGG-Sal1-F | BIOTIN-GTAAGCTGGAAGTTGTTGCTGCTGCCTGAGACTGGAGGATCTGTCGAC |
| NESTSpyTag-SpeI-R3 | GCCTCCCATAGAACTAGTAGATCCCTTAGTC |
| NESTOLS79-duetMCS2-F | GAAGTTGTTGCTGCGTGAGCGGAT |
| NESTOLS79-LPETGG-F | GAAGTTGTTGCTGCTGCCTGAGAC |
| NESTSpyTag-SpeI-R4 | TCCCATAGAACTAGTAGATCCCTTAGTCGG |
| spyT-F19 | CAAGCAGAAGACGGCATACGAGATGCCAATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTAGCTGTATATTAGTTAAGTATAAGAAGGAGATATACATATG |
| spyT-R19 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTAGCTCATAGAACTAGTAGATCCCTTAGTCGG |
| spyT-F17 | CAAGCAGAAGACGGCATACGAGATGCCAATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTACGTGATATACATATGGGAGCTCAC |
| spyT-R17 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTACGTAGATCCCTTAGTCGGCTTATATGCGTC |