Figure 6.

(A) ¹H,¹H-NOESY, (B) ¹H,¹⁵N-TROSY and (C) HNN-COSY spectra for imino-proton correlations of the 5′-genomic end construct 5_SL5stem encompassing nts 150–180 and 265–294. Positive contours are given in blue, negative contours in red. The imino-proton correlations are annotated using the genomic numbering. Sequential imino-proton correlations in (A) between neighboring base pairs are shown in colors according to the experimentally determined 5_SL5stem RNA secondary structure schematized as an inset in panel (C). NMR resonances arising from the tetraloop nucleotides are annotated with small letters and the two additional GC closing base pairs at the 5′- and 3′-termini are numbered with ±x, respectively. Colors of boxes are according to the correlations in (A). Note that the stem is depicted upside down with respect to its orientation as presented in the overall scheme of Figure 1.