Figure 8.

(A) ¹H,¹H-NOESY, (B) ¹H,¹⁵N-TROSY and (C) HNN-COSY spectra for imino-proton correlation of the 5′-genomic end construct 5_SL5b+c encompassing nucleotides 227 to 263 at 283K. Positive contours are given in blue, negative contours in red. The imino-proton correlations are annotated using the genomic numbering. Imino-proton correlations in (A) between consecutive base pairs are shown in different colors, * denote crosspeaks visible at 275K, but not at 283K. Included in (C) is the experimentally observed secondary structure of 5_SL5b+c with genomic numbering. Additional closing base pairs are annotated with ‘±x’. The boxes are according to the correlations in (A).