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RAD521–209 promotes ssDNA annealing (SSA) in U2OS cells. (A) The scheme of the SSA-GFP reporter system. (B and C) Efficiency of SSA in U2OS cells with endogenous RAD52 knocked out and indicated exogenous RAD52 constructs were expressed. Error bars indicate S.D. (n = 3) and statistical analysis was performed using one-way ANOVA with Tukeys multiple comparison test; ns P > 0.05, **P ≤ 0.01 and ***P ≤ 0.001.
