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. 2020 Dec 4;48(22):12778–12791. doi: 10.1093/nar/gkaa1145

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — RAD52_1–209 promotes homology dependent repair (HDR) of DSBs by gene conversion in BRCA1-deficient MDA-MB-436 cells. (A) The scheme of the HDR assay. (B) Efficiency of I-Sce induced HDR in MDA-MB-436 BRCA1^+/+ and MDA-MB-436 BRCA1^−/− treated with shRAD52 or a control shRNA (shCtrl). The relative change of HDR efficiency for each group was calculated by normalizing each to the HDR efficiency obtained for BRCA1^+/+ cells. (C) Efficiency of I-Sce induced HDR in BRCA1^−/− cells expressing RAD52_WT or RAD52_1–209 or empty vector treated with shRAD52 or control shRNA(shCtrl). (D) Efficiency of I-Sce induced HDR in BRCA1^−/− cells expressing RAD52_WT or RAD52_YI65–66AA or RAD52_K102A/K133A or empty vector treated with shRAD52.The relative change of HDR efficiency for each group in panels (C) and (D) was calculated by normalizing to the HDR efficiency obtained for cells expressing empty vector and shCtrl. (E) Efficiency of I-Sce induced HDR in BRCA1^−/− cells expressing RAD52_1–209 with shRAD52 and treated with D-I03 (50 μM) or DMSO (1%). Data normalized to the HDR efficiency obtained for cells treated with DMSO. Error bars represent S.D (n = 3), statistical analysis was performed using One-way ANOVA with Tukeys multiple comparison test in (B), (C) and (D) and Two-way ANOVA with Bonferroni's multiple comparisons test in (E); **P ≤ 0.01, ^****P ≤ 0.0001 and ns P > 0.05.