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. 2020 Nov 5;48(22):e132. doi: 10.1093/nar/gkaa987

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Schematic representation of microdroplet DNA sequencing technology. Droplets containing reagents required for pyrophosphorolysis (PPL) are passed over the DNA strand to be sequenced, which is bound to an immobilized microsphere. These droplets, some of which will now contain a dNTP released through PPL, are individually merged with droplets containing reagents to detect the presence and type of each dNTP. Capture of a dNTP produces a strong fluorescent signal triggered by an individual nucleotide. Type of fluorescent signal indicates the nature of the dNTP and the order of the microdroplets corresponds to the sequence.