Figure 2.

Optimization of AzG-labeling conditions. (A) Confocal fluorescence microscopy images showing E. coli treated with AzG at varied concentrations for 2 h, followed by click reaction with alkyne-Cy5. Scale bars, 2 μm. (B) In-gel fluorescence showing total RNA from E. coli treated with 100 μM AzG for varied durations of time. (C) In-gel fluorescence scanning showing total RNA that were isolated from E. coli treated with 100 μM AzG at varied starting cell concentrations, and reacted with DBCO-Cy5. In (B) and (C), GelRed-stained gels demonstrate equal loading.