Figure 3.

LC/MS analysis of global 2′-O-methylation content in total Escherichia coli tRNA fraction. Normalized LC/MS content of modified nucleoside is shown for 2′-O-guanosine (Gm), 2′-O-cytosine (Cm), 2′-O-uridine (Um) and pseudouridine (used as control here). Biological replicates (n = 3) and technical replicates (n = 2) are shown as color bars and black dots/triangles/squares, respectively. *: P-value < 0.05, **: P-value < 0.01, ****: P-value < 0.0001. Statistical values were calculated for technical and biological replicates using two-tailed t-test with unequal variance.