Table 1.

mES cell replicon characteristics

Experimental data	Mean ± SEM
RFS, 1000 nts per min	1.67 ± 0.02
IOD, kb	90 ± 3.6
Genome size (GS), 1000 Mb	5.19*–6.5**
Active RFi at any given point during S-phase	3 320 ± 20
Total S-phase duration, minutes	654 ± 21
Single forks, %	12.81 ± 8.6
Calculations	Mean
Time to replicate the genome with one fork, (GS/RFS), hours	51 796–64 870
Replication forks active in parallel, (GS/RFS/S-phase duration)	4 752–5 951
Replicons active in parallel, (active forks/2)	2 376–2 976
Replicons per RF, (calculated replicons active in parallel/counted RFi)	0.72–0.9
Calculated % of single forks	10–28

RFS, replication fork speed; nts, nucleotides; IOD, inter-origin distance; GS, genome size (based on published genome data); RF, replication focus; RFi, replication foci; SEM, standard error of the mean; * GS according to mouse reference genome (GRCm38); ** GS according to flow cytometry measurements.