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. 2020 Dec 7;13:4809–4822. doi: 10.2147/DMSO.S281527

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© 2020 Shaher et al.

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Effect of Ganoderma lucidum spore (GLS) on brain natriuretic peptide (BNP, molecular weight is 37 kDa) and α-smooth muscle actin (α-SMA, molecular weight is 42 kDa) expression in myocardial tissues. Representative Western blot images (A) and quantitative analysis for expression of BNP (B) and α-SMA (C) in the myocardial tissues of rats from different groups. Representative immunohistochemistry micrographs for BNP expression in cardiac tissues of rats from different groups (D). Strong BNP immunostaining was observed in most of the myocardial cells in the STZ group, compared with the control and STZ +GLS groups (see the arrow-indicated area). Statistical analysis of immunohistochemistry results for cardiac BNP expression (E). Values represent mean ± SE; n = 3 in each group. **, p < 0.01 versus the control group and ^##, p < 0.01 versus the STZ group using Tukey’s test. Control: 5 mL/kg saline (p.o.); Diabetic: 50 mg/kg streptozotocin (i.p.) and 5 mL/kg saline (p.o.); STZ +GLS: 50 mg/kg streptozotocin (i.p.) and 300 mg/kg GLS (p.o.).