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. 2020 Dec 10;12:12697–12708. doi: 10.2147/CMAR.S277113

Figure 3.

Figure 3

LINC01426 is a molecular sponge for miR-519d-5p in NSCLC cells. (A) The subcellular localization of LINC01426 was predicted by lncATLAS. (B) Nuclear–cytoplasmic fractionation assay was conducted to evaluate the distribution of LINC01426 in H460 and A549 cells. (C) The putative miRNAs that interact with LINC01426 were predicted by miRDB. (D) miR-519d-5p, miR-873-3p, miR-377-5p, and miR-548c-3p expression levels in H460 and A549 cells after LINC01426 knockdown was determined using qRT-PCR. (E) qRT-PCR was performed to detect miR-519d-5p expression in 58 pairs of NSCLC tissues and corresponding adjacent normal tissues. (F) Pearson’s correlation analysis was conducted to address the correlation between LINC01426 and miR-519d-5p expression in the 58 NSCLC tissues. (G) The putative binding sequence of miR-519d-5p within the sequence of LINC01426 was determined via a bioinformatics analysis, and the mutant binding sequences are shown. (H) The transfection efficiency of the miR-519d-5p mimic in increasing endogenous miR-519d-5p expression in H460 and A549 cells was measured using qRT-PCR. (I) Luciferase activity was measured in H460 and A549 cells following miR-519d-5p mimic or miR-NC transfection and LINC01426-wt or LINC01426-mut transfection. (J) RIP assay was employed to assess the enrichment of miR-519d-5p and LINC01426 in the anti-Ago2 or anti-IgG precipitates. **P < 0.01.