Fig. 5. Silencing NRF1 reverses PA28α knockdown-induced proteasome recovery and resensitizes to bortezomib treatment.

a Western blot analysis of NRF1 in LP1 and RPMI8226 PA28α knockdown and control stable cells. b LP1 PA28α knockdown and control stable cells were transiently transfected with NRF1 siRNA or control siRNA. 48 h after transfection, cell lysates were collected and analyzed for expression of proteasome subunits by western blot (b) or cells were treated with different concentrations of bortezomib for 24 h, MTT assay was performed to assess relative cell growth (c).