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. 2020 Dec 14;6:14. doi: 10.1038/s41514-020-00053-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 12 — Immunofluorescence detection of 8-OHdG in the SGNs (a, c) and hair cells (b, d) at each turn in the Keap1^FA/FA and WT mice at 5 (a, b) and 12 (c, d) months of age. 8-OHdG signals are shown as red fluorescence. Nuclei were counterstained with DAPI (blue). OHCs, outer hair cells; IHC, inner hair cell. The experiments were performed for five samples in each group. Relative intensities of the 8-OHdG signals at each turn were semi-quantified. Average staining intensities of WT mice are set as 1. The data represent the mean ± SEM. *P < 0.05. Unpaired two-tailed Student’s t-test was applied. Scale bars correspond to 50 μm (a, c) and 20 μm (b, d).