Table 1.
Binding affinities of the test compounds.
| Ki [nM]a | ||||
|---|---|---|---|---|
| [3H]spiperone | ||||
| hD2LR | hD2SR | hD3R | hD4R | |
| 2a | 8.7 ± 2.5 (4) | 6.5 ± 1.7 (4) | 0.41 ± 0.09 (4) | 33 ± 7 (4) |
| 2b | 15 ± 5 (3) | 6.3 ± 0.2 (3) | 1.0 ± 0.2 (3) | 120 ± 61 (3) |
| 2c | 77 ± 17 (3) | 34 ± 8 (3) | 13 ± 4 (3) | 140 ± 10 (3) |
| 2d | 360 ± 60 (4) | 350 ± 30 (4) | 9.5 ± 1.9 (4) | 5,000 ± 300 (4) |
| 4a | 5.2 ± 0.6 (4) | 1.7 ± 0.3 (5) | 0.29 ± 0.02 (5) | 27 ± 3 (5) |
| 4b | 20 ± 8 (3) | 5.1 ± 1.7 (3) | 1.9 ± 0.7(3) | 59 ± 20 (3) |
| 4c | 17 ± 7 (3) | 8.4 ± 3.4 (3) | 6.5 ± 2.3 (3) | 32 ± 6 (3) |
| 4d | 7,600 ± 3,000 (4) | 6,000 ± 1,400 (4) | 11 ± 2 (4) | > 10,000 (4) |
| 7a | 3.5 ± 0.4 (8) | 1.8 ± 0.2 (8) | 0.26 ± 0.03 (8) | 8.5 ± 0.8 (8) |
| 8a (NMP64) | 1.2 ± 0.2 (4) | 0.44 ± 0.12 (4) | 0.32 ± 0.05 (4) | 10 ± 4 (4) |
| 8b | 13 ± 6 (4) | 3.6 ± 1.6 (4) | 1.9 ± 0.9 (4) | 130 ± 10 (4) |
| 10a (NMP160) | 46 ± 7 (4) | 21 ± 4 (4) | 0.97 ± 0.23 (4) | 56 ± 4 (4) |
| 10b (NMP137) | 10 ± 2 (5) | 4.8 ± 1.0 (5) | 0.90 ± 0.26 (5) | 50 ± 7 (4) |
| 10c (NMP130) | 23 ± 5 (4) | 15 ± 4 (4) | 0.76 ± 0.11 (4) | 47 ± 9 (4) |
Binding affinities of the test compounds for human D2LR, D2SR, D3R, D4R were determined by radioligand competition.
aData represent mean ± SEM of (n) individual experiments, each performed in triplicates.