Fig. 4. Identification of SlLCD1 gene-edited plants.

A Gene structure of SlLCD1, which is indicated as the 5’-UTR (untranslated region) and CDS (coding sequence). Generation of SlLCD1 mutations by CRISPR/Cas9 using single-guide RNAs (sgRNAs). Sequences of the SlLCD1 mutants lcd1-7 and lcd1-9 are shown. sgRNA target and a protospacer-adjacent motif (PAM) are indicated in red and green, respectively. Deletions and insertions are indicated by dashes. B Endogenous H₂S content in leaves of the wild type (WT) and the lcd1-7 and lcd1-9 mutants of tomato. The values are the means ± SDs of three replicates. The symbol ** stands for P < 0.01