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. 2020 Dec 14;11(12):1070. doi: 10.1038/s41419-020-03275-2

Fig. 4. MB-PDT activates necroptosis in tumorigenic breast cells.

Fig. 4

A MCF-10A, B MCF-7, and C MDA-MB-231 cells were submitted or not to MB-PDT. One, 3, and 24 h after irradiation, cells were lysed and protein extracts were submitted to western blotting. Representative images of western blottings of pMLKL, MLKL, and tubulin, and the corresponding quantification of pMLKL/MLKL ratio are shown. *p < 0.05 vs. Control (n = 4 independent experiments). D MCF-10A, E MCF-7, and F MDA-MB-231 were pretreated or not with Nec-1 or NSA, and then submitted or not to MB-PDT. Cell death was evaluated 1, 3, or 24 h after irradiation. ***p < 0.001, **p < 0.005, *p < 0.05 vs. MB-PDT. Dot colors representation: MCF-10A in red, MCF-7 in green, and MDA-MB-231 in blue. Results are presented as mean ± SEM (n = 3 independent experiments).