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. 2020 Dec 14;10:21837. doi: 10.1038/s41598-020-78651-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression of inflammasome-related genes. Human neutrophils were infected with UPEC strain CFT073 at MOI 1 or MOI 10 for 3 h (A) and 6 h (B) followed by analysis of mRNA expression. The gene expression was normalized to GAPDH and expressed as fold change relative to unstimulated controls. Data are presented as mean ± SEM (n = 3 independent experiments) with the y-axis as Log2. Bars below 1 (the dotted line) indicate decreased gene expression. Asterisks denote statistical significance compared to respective unstimulated control (*p < 0.05, **p < 0.01, ***p < 0.001).