Figure 1.

MR1T Cells Are Present in Circulation and Tissues of P. alecto

(A) Representative histogram of Pa bone marrow (BM) mononuclear cells (MNCs) either pre-blocked with titrated concentrations of hMR1-6-FP or hMR1-5-OP-RU tetramer as indicated, followed by incubation with a fixed concentration of hMR1-5-OP-RU tetramer (top panel) or hMR1-6-FP tetramer (bottom panel), respectively (n = 3).

(B) The mean fluorescent intensity (MFI) levels of hMR1-5-OP-RU tetramer following hMR1-6-FP pre-blocking, and hMR1-6-FP teramer following hMR1-5-OP-RU pre-blocking (n = 3). See also Figures S2A–S2C.

(C and D) (C) Identification and (D) enumeration of MR1T cells in the circulation, spleen (spl), and BM of Pa (n = 6-14). Human peripheral blood (PB) MAIT cells were included as a comparison (n = 57). See also Figures S2D and S2E.

(E and F) (E) Representative histrograms and (F) expression of the transcription factors PLZF, RORγt, T-bet, and Eomes as well as the cytolytic protein perforin (Prf) in MR1T cells and non-MR1T cells in BM tissues of Pa bats (n = 7-12) and in human MAIT cells. See also Figure S2F. Data presented as a line graph with error bars represents the mean and standard error. Box and whisker plots show all data points, median, and the interquartile range. See also Figure S2. Statistical significance was determined using the Kruskal-Wallis ANOVA followed by Dunn's multiple comparison test followed post-hoc test (D), Wilcoxon's signed-rank test (E; PLZF), or paired t test (F; RORγt, T-bet, Eomes, Prf). ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001,∗∗p < 0.01,∗p < 0.05. ns, not significant.