Figure 4.

Unprimed Pa MR1T Cells Were Incapable in Producing TNF and IL-17 following Stimulation with Agonist MR1 ligands.

(A and B) (A) Concatenation and levels (B) of Pa MR1T or hMAIT cell hMR1-5-OP-RU staining intensity (MFI) from following stimulation with 5-OP-RU, riboflavin synthesis-competent E. coli, or PMA/ionomycin (n = 6 each).

(C) Comparison of hMR1-5-OP-RU staining intensity (MFI) between Pa MR1T cells and hMAIT cells following stimulations relative to unstimulated controls (n = 6). (D) Representative flow cytometry plots of (E) TNF and (F) IL-17 expression by freshly thawed (day 0) Pa BM MR1T cells or hMAIT cells following 24 h culture with MR1 ligand 5-OP-RU or E. coli EC120S (n = 6). PMA/ionomycin treatment for 6 h were used as positive controls. (G) TNF and IL-17 production by freshly thawed Pa BM MR1T cells at indicated time points following incubation with MR1 ligand 5-OP-RU or E. coli EC120S (n = 2-6). See also Figures S3A–S3D. Data presented as a line graph with error bars represent the mean and standard error. Box and whisker plot shows all data points, median, and the interquartile range. Statistical significance was determined using the Friedman test followed with Dunn's multiple comparison test (B), and Mann-Whitney's test (C, E, and F). ∗∗p < 0.01,∗p < 0.05, [∗] p < 0.1. ns, not significant.