Figure 2.

LINC00162 can Promote the Proliferation of Bladder Cancer Cells

(A) LINC00162 KEGG pathway analysis. The vertical axis represents the pathway. The horizontal axis represents the enrichment factor (ratio of the number of annotated genes in the corresponding pathway rich in differentially expressed genes). The size of the dots indicates the number of differentially expressed genes in the corresponding pathway (number of genes). The color of the dots indicates the range of p values.

(B) The 5-ethynyl-2’-deoxyuridine (EdU) assay was used to detect the role of LINC00162 in bladder cancer cell proliferation, and the scale bar represents 300 μm∗p < 0.05.

(C) The role of LINC00162 in the activity of bladder cancer cells was detected by the ATP method, ∗p < 0.05.

(D) Flow cytometry was used to detect the role of LINC00162 in bladder cancer cell apoptosis, ∗p < 0.05.

(E) The function of LINC00162 in bladder cancer cell cycle was tested by flow cytometry. Experimental cells were stained with propidium iodide (PI). Cell counts represent the number of cells at different cell cycle stages, ∗p < 0.05.

(F) The results of the soft agar experiment (UM-UC-3-vector and UM-UC-3-LINC00162-shRNA), and the scale bar represents 200 μm, ∗p < 0.05.

(G) Subcutaneous tumor-bearing results in nude mice at 27 days, ∗p < 0.05.