Fig. 1.

Single-particle cryo-EM analysis of in-situ structure of the HCoV-NL63 coronavirus spike glycoprotein. (a) Representative motion-corrected cryo-EM micrograph. (b) Reference-free 2D class averages of computationally extracted spikes. (c-d) Reconstructed 3-fold symmetry-imposed cryo-EM map of the spike in the top and side views without (c) and with (d) glycans shown. (e) Resolution variation maps for 3D reconstruction. Left, whole map view; middle, slice view; right, whole map view at a lower threshold.