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. 2020 Nov 18;3(6):1100–1110. doi: 10.1021/acsptsci.0c00073

Figure 6.

Figure 6

AD16 alters lysosomal distribution in BV2 microglial cells. BV2 cells were treated with or without AD16 for 24 h in serum-free Opti-MEM medium incubated with LPS (100 ng/mL). Lysosomal distributions were detected through the staining of LAMP1 in BV2 microglial cells treated with AD16 or vehicle (A). Quantification of LAMP1 distribution in BV2 microglial cells (n = 3) (B). Immunoblot assays against LAMP1 protein are shown (C). Statistical data of the expressions of LAMP1 from three independent experiments are shown (n = 3). Protein blots were analyzed using ImageJ and bands were normalized to their respective β-actin loading controls. Data are representative of the average fold change with respect to control for three independent experiments. (D). ATP levels were detected in LPS-activated BV2 cells with AD16 or vehicle (n = 3) (E). Scale bar: 20 μm. Statistical comparisons were performed by two-way ANOVA. Post hoc group-wise comparisons were conducted using the Sidak’s multiple comparisons test. Data are presented as means ± SD; *p < 0.05, **p < 0.01.