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. 2020 Oct 7;3(6):1253–1264. doi: 10.1021/acsptsci.0c00125

Figure 4.

Figure 4

Selective death of osteosarcoma with short telomeres following treatment with ATR inhibitor VE-822. Short telomere cell lines, OHSN, HOS-MNNG, and HAL, and long telomere cell lines, MG-63 and MHM, treated with VE-822 were assessed using IncuCyte live cell analysis. Medium was supplemented with the live-cell-impermeable dye SYTOX to quantify cell death. (a) Representative images of OHSN and MG-63 treated with vehicle (DMSO) or 1 μM VE-822 at the indicated time. A 15 μm scale bar is shown. Phase contrast images are overlaid with SYTOX death dye signal (red) marking dead cells. (b–f) Representative graphs depicting net death over time, reflected by SYTOX death dye incorporation normalized to cell density for OHSN (b), HOS-MNNG (c), HAL (d), MG-63 (e), and MHM (f). Data representative of two or more experiments.