Table 1. Telomere Status of the Osteosarcoma Cell Lines.
| cell line | telomere statusa | qPCR T/S ratiob | median telomere length (kb)c | CC assay level | TERC leveld | TERT mRNA leveld |
|---|---|---|---|---|---|---|
| HOS-MNNG | ST | 0.813 | 3.0 | –0.006 | 19.80% | 56.54% |
| OHSN | ST | 0.837 | 3.2 | –0.011 | 23.17% | 107.37% |
| SJSA-1 | ST | 0.840 | 3.2 | –0.002 | 6.36% | 695.03% |
| HAL | ST | 0.853 | 2.7 | 0.007 | 1.48% | 170.34% |
| 143b | ST | 0.855 | 3.0 | 0.002 | 26.48% | 288.96% |
| HOS | ST | 0.902 | 3.6 | 0.001 | 21.03% | 323.13% |
| MG-63 | LT | 0.966 | 5.8 | –0.003 | 11.91% | 58.47% |
| MHM | LT | 1.086 | 6.4 | 0.001 | 29.03% | 390.11% |
| HuO-3N1 | LT | 1.129 | 6.7 | 0.029 | 35.41% | 7.69% |
| G292 | ALT | 1.415 | 10.7 | 4.156 | 3.98% | 0.06% |
| HuO-9 | ALT | 1.539 | 9.7 | 1.066 | 3.69% | 1.33% |
| CAL72 | ALT | 2.853 | 10.2 | 5.422 | 5.30% | 2.22% |
| U2OS | ALT | 4.221 | 13.7 | 0.835 | 0.00% | 4.21% |
| KPD | ALT | 1.210 | 10.0 | 2.944 | 0.01% | 4.42% |
| NY | ALT | 1.552 | 9.9 | 0.651 | 0.77% | 14.54% |
| SAOS-2 | ALT | 1.230 | 7.6 | 1.975 | 21.87% | 19.36% |
| LM7 | ALT | 1.170 | 8.2 | 1.227 | 12.27% | 0.29% |
a
ST, ALT-negative, short-telomere. LT, ALT-negative, long-telomere. ALT, ALT-positive.
b
T/S, telomere repeats to single-copy genes.
c
Calculated using Telometric software.
d
Relative to HEK293T expression, determined as expression fold change versus 7SK.