Fig. 2. Expression dynamics and functions of genes in three gene ontology (GO) terms.

Genes that were differentially expressed between 10.5 months post immunization and day 0 were submitted to DAVID database to identify those associated with the five GO terms enriched in “memory specific clusters” in Fig. 1b. Global expression heatmaps of genes associated with GO terms “cell proliferation” (a), “DNA repair” (b) or “apoptosis” (c) were created with a row Z-Score ranges from −4 to 4(left panel). A few representative genes from each GO term gene group were selected with fold changes in their expression levels at different time points comparing to day 0 (middle panel). The potential function and related pathways of these selected genes were depicted (right panel). Genes were differentially (fold change > 2, p-value < 0.05) upregulated (pink box), downregulated (blue box) or not differentially expressed (white box) in 10.5 months post immunization compared to day 0. Genes in red circle have been shown in literature to be able to form complexes. Arrows between genes/pathways indicate positive regulation and lines with blunt end between genes/pathways indicate negative regulation by literature references.