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. 2020 Dec 1;11:580719. doi: 10.3389/fmicb.2020.580719

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Copyright © 2020 Hunter, Paight and Lane.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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System overview of Cardiosporidium cionae and Nephromyces showing tunicate host (A,F), area of localization (B,G), filamentous life stage (C,H), oocyst life stage (D,I), and vertically transferred fluorescent in situ hybridization (FISH) labeled bacterial endosymbionts within the oocysts (E,J). Scale bars are approximations due to resizing of images. FISH was carried out according to the method in Paight et al. (2020).