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. Author manuscript; available in PMC: 2020 Dec 15.
Published in final edited form as: Cell Immunol. 2020 Sep 9;357:104201. doi: 10.1016/j.cellimm.2020.104201

Fig. 3.

Fig. 3.

TLR-ligation of HSPCs did not grossly affect granulocyte maturation in vitro. HSPCs were stimulated with various TLR ligands for 6 days prior to seeding into semisolid methylcellulose media (1000 cells/well). (A)Total colony numbers are presented, (B) with numbers of erythroid burst forming units (BFU-E), erythroid colony forming units (CFU-E), monocyte colony forming units (CFU-M), granulocyte-monocyte colony forming units (CFU-GM), and granulocyte-erythrocyte-megakaryocyte-monocyte colony forming unit (CFU-GEMM). (C) Flow cytometric analyses of harvested colonies indicate total myeloid numbers, (D) frequencies of ARID3a+ myeloid cells, and numbers of (E) CD33+ CD14+ CD16+ monocytes, (F) CD33+ CD14− CD16+ granulocytes, and (G) CD41+ megakaryocytes. Means and standard errors are indicated. Significance was assessed by paired T test, *, p < 0.03, **, p < 0.005.