Skip to main content
. 2020 Dec 14;11:542. doi: 10.1186/s13287-020-02069-9

Fig. 5.

Fig. 5

Characterization of neuronal cells. ac Characterization of the neuronal cells with Cresyl violet staining. The differentiated cells from hSCAPs and RSV-hSCAPs revealed intense purple substance at cell body (white arrow), while the crt-hSCAPs showed the pale purple background of the nucleus (white asterisk) and dark violet of the nucleolus. Scale bars: a, b, and c = 100 μm. df The intracellular calcium oscillation of crt-hSCAPs, d-hSCAPs, and RSV-d-hSCAPs. The intensity of calcium has showed to be slightly increased and suddenly reached the highest peak in both d-hSCAPs and RSV-d-hSCAPs, while the hSCAPs exhibited steady patterns as a baseline intensity (n = 3). g The graph plotted between Ca2+ intensity and time (minutes) of crt-hSCAPs (gray line), d-hSCAPs (orange line), and RSV-d-hSCAPs (blue line)