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. 2020 Dec 14;21:892. doi: 10.1186/s12864-020-07312-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The relative expression levels of the pNP genes in U. unicinctus larvae during the settlement. a, A time course of U. unicinctus development indicating qRT-PCR sampling strategy employed in this study. b, the relative expression levels of the pNP genes. LT, late-trochophore (25 dpf, pelagic larva); PL, pre-competent larva (32 dpf, correspond to ES in transcriptome data); CL, competent larva (35 dpf, correspond to SL in transcriptome data); POL, post-competent larva (38 dpf); WL, worm-shaped larva (42 dpf, benthic larva). Data are indicated as mean ± SD from triplicate experiments and analyzed using One-way ANOVA followed by Tukey’s HSD test. Different letters indicate significant difference between different developmental stages (p < 0.05)