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. Author manuscript; available in PMC: 2020 Dec 15.
Published in final edited form as: J Biophotonics. 2020 Jul 28;13(10):e202000090. doi: 10.1002/jbio.202000090

FIGURE 5.

FIGURE 5

Visual summary (A-D) and proposed explanation (E) for study results. (A-D) After Intralipid injection, signals in connecting microvessels are preferentially enhanced, as shown in overlay (D) of signal contributions from RBCs (red) and Intralipid tracer (green). (E) Intralipid particles are smaller than RBCs and spherically symmetric. Thus, Intralipid backscattering in OCTA does not depend on microvessel orientation, and Intralipid signal serves as a convenient reference. We propose that longitudinal RBC elongation contributes to the lower relative RBC signal in connecting microvessels. Due to this elongation, incident light sees a smaller effective cross section for RBCs in diving or ascending microvessels than for RBCs in en face capillaries, resulting in relatively less OCTA signal