FIGURE 5.

Impact of disease status on endocannabinoid system gene expression analysis in human airway epithelial cells from healthy individuals, people with asthma, and individuals with COPD. a) Principal-component (PC) analysis plot of healthy subjects (n=616), people with asthma (n=136) and individuals with COPD (n=338) generated by expression patterns of the 32-gene endocannabinoid signature. The first (18%) and second (16.3%) PCs were used. Ellipses were added to represent 95% confidence intervals per sex. b) Gene expression data of the 32 genes were compared between healthy, asthmatic and COPD samples. The log₂-transformed mean expression values were compared to that of the healthy samples and shown as log₂ fold change (FC). Visual representation of the differences in the 32-gene endocannabinoid signature between c) healthy subjects and people with asthma and d) healthy subjects and individuals with COPD. Colour coding is reflective of log₂FC relative to healthy subjects. THC: tetrahydrocannabinol. *: p<0.05; **: p<0.01; ***: p<0.001.