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Crystal structure of GAOV and superposition with WT GAO (1GOG). The three domains of GAOV are presented in red, blue, and green with WT GAO in grey. Residues of directed evolution mutations are illustrated in stick form. The inset on the top right shows the active site of the cross-linked form GAOV, and the omit Fo-Fc electron densities (blue) is contoured at 4.0 σ. The inset on the bottom right shows the comparison of the active sites of both structures.
