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The genetic incorporation of an unnatural tyrosine into GAOV. (A) The slower moving band corresponding to uncrosslinked protein (U) and faster moving band corresponding to the mature GAOV (C). The lanes are molecular marker (M), GAOV (1), F2-Tyr272 GAOV (2), and Cl2-Tyr272 GAOV (3), respectively. (B) The crosslinked and uncrosslinked chemical structures (X=H/F/Cl).
