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. Author manuscript; available in PMC: 2021 May 2.
Published in final edited form as: J Vis Exp. 2020 Nov 2;(165):10.3791/61791. doi: 10.3791/61791

Figure 1: Representative confocal microscopy images of breast epithelial spheroids.

Figure 1:

MCF10A spheroids were labeled for integrin α6 (green) and nuclei (blue). Cell phenotype can be confirmed after bioprinting when spheroids appear round with a hollow center (inset) and have integrin α6 polarized at the outer edges. MDA-MB-231 spheroids were labeled for actin (green) and nuclei (blue). Cell phenotype can be confirmed when spheroids are irregularly shaped without hollow centers and have cell processes invading into the surrounding matrix. Scale bar = 50 μm.